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Corresponding Author

Mohamed Abdelhamid Elsayed Elhadidy

Document Type

Original Article

Abstract

Background: Recurrent pregnancy loss (RPL) remains a challenging concern for women of childbearing age. Genetic mutations, particularly prothrombin G20210A and factor V Leiden, have contributed to RPL. Understanding their impact on pregnancy outcomes is crucial for tailored interventions.

Aim: To determine the possible mutation of Factor V Leiden and the Prothrombin G20210A mutation by reverse hybridization technique encountering RPL.

Methods: This case-control research was executed on 100 patients (50 experiencing RPL and 50 controls). Patients underwent thorough clinical evaluations and genetic analyses to detect thrombophilia gene variants, including PCR and sequencing techniques. Laboratory assessments encompassed various coagulation factors and protein levels.

Results: Significantly higher occurrences of stillbirth/neonatal death were observed in the patient group (71.4%) in contrast to controls (98.0%). The patient group showed a median of three successful pregnancies (vs. 0.5 in controls) and a higher median number of abortions (20 vs. 3 in controls). Additionally, gestational age at abortions was notably higher in the patient group (9.22 weeks vs. 8.08 weeks in controls). The patient group exhibited significant differences in protein S free antigen, protein C antigen, antithrombin III activity, and protein S activity compared to controls. Factor V Leiden heterozygosity was found in 68.0% of patients, while Prothrombin G20210A heterozygosity was present in 24.0%.

Conclusions: It is not comparable to compare pregnancy loss and live birth rates, stillbirths and neonatal deaths in females with or without mutations in Prothrombin or Factor V Leiden.

Keywords

Recurrent Pregnancy Loss; Factor V Leiden; Prothrombin G20210A; Genetic Mutations; Pregnancy Outcomes

Subject Area

Obstetrics and Gynecology

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