•  
  •  
 

Corresponding Author

Esam, Yomna

Document Type

Original Article

Abstract

Magnetic activated cell sorting (MACS) has mechanisms to reduce the degrees of Deoxyribonucleic acid (DNA) fragmentation done to the sperm sample in sperm processing pre-injection since no density gradient is involved or heavy centrifugation steps involved. If DNA fragmentation is not eliminated from sperms, poor cleavage rate, bad embryo quality, poor implantation and low pregnancy rates may occur. A MACS is Nano-sized mixture of particles with high magnetic properties coated with glycoproteins for morbid/DNA fragmented Spermatozoa depletion prior to Intra Cytoplasmic Sperm Injection (ICSI). Several studies have shown that the using of MACS technique improve the outcomes of ICSI in patients with high levels of sperm DNA fragmentation. We aimed to prove that the assessing Utility of Clemente Magnetic Activated Cell Sorting in ICSI cycle (especially recurrent implantation failure) would improve cleavage rate, Embryo quality, implantation and hopefully pregnancy rates. We determined if MACS is suitable for Male factor with high DNA Fragmentation Index (DFI) undergoing ICSI or In vitro fertilization (IVF), recurrent miscarriage that can be attributed to male factor and early pregnancy loss attributed to male factor. We evidenced that the using of MACS techniques in ICSI resulted in increasing fertilization rate, cleavage rate, blastocyst formation rate, number of Grade A embryos and pregnancy rates, as the using of MACS in sperm processing helps in increasing motility and especially progressive motility, reduce sperms with abnormal morphological defects.

Keywords

MACS; sperm DNA fragmentation; ICSI; Male infertility

Download

Share

COinS