Evaluation of IL28B Gene Sequence Variations in the Diagnosed Null-Response to DAAs Therapy by Intra PBMCs Nested HCV RNA PCR

Authors

Mohamed Abd Alla, Department of Hepatology, Gastroenterology and Infectious Diseases. Faculty of Medicine, Al-Azhar UniversityFollow
ahmed altambouly, hepatology &gastroenterology and infectious diseases alazhar university CairoFollow
Mohamed Abd Alla, Department of Hepatology, Gastroenterology and Infectious Diseases. Faculty of Medicine, Al-Azhar UniversityFollow
Galal AbuFarrag, Department of Hepatology, Gastroenterology and Infectious Diseases. Faculty of Medicine, Al-Azhar UniversityFollow
Islam Ammar, Department of Hepatology, Gastroenterology and Infectious Diseases. Faculty of Medicine, Al-Azhar UniversityFollow
Mostafa El-Awady, Molecular Genetics- Department of Microbial Biotechnology National Research center – Cairo EgyptFollow

Corresponding Author

Abd Alla, Mohamed

Document Type

Original Article

Abstract

Background: Single nucleotide polymorphisms (SNPs) of the IL-28B gene are reliable predictors of chronic HCV disease course after treatment with Peg-IFN and Ribavirin (RBV). Aims, Subjects and Methods: Impacts of IL-28B-SNPs on the response to direct acting antivirals (DAAs) were evaluated in posttreatment negative serum and cellular HCV-PCR (group I), solitary intra-PBMCs viral infections (group II), and positive HCV-RNA serology (group III). Twelve weeks after completing DAAs-therapy, 45 patients were recruited and divided into three groups (n=15each), as afro-mentioned. IL-28B gene sequencing was done to detect SNPs in relation to hepatic parenchymal changes in the selected study populations. Results and conclusion: Changes in liver parenchyma of group III (46.7%) and II (26.6%) were associated with higher HCV-relapse compared to I (P

Keywords

IL28B gene; PBMCs PCR; DAAs; HCV Relapse

How to Cite This Article

Abd Alla, Mohamed; altambouly, ahmed; Abd Alla, Mohamed; AbuFarrag, Galal; Ammar, Islam; and El-Awady, Mostafa (2020) "Evaluation of IL28B Gene Sequence Variations in the Diagnosed Null-Response to DAAs Therapy by Intra PBMCs Nested HCV RNA PCR," Al-Azhar International Medical Journal: Vol. 1: Iss. 11, Article 11.
DOI: https://doi.org/10.21608/aimj.2021.40774.1313