Abstract
Background: One of the main sources of acquired bacterial infections in the community and hospitals is Staphylococcus aureus, particularly MRSA strains.
Aim of the work: To compare between the CHROMagarTM MRSA selective medium (as a rapid method) and the other laboratory conventional methods. Also, to evaluate of the presence of mecA gene within MRSA isolates using Polymerase chain reaction (PCR).
Materials and Methods: The samples were screened for antibiotic susceptibility testing and growth on a particular chromogenic medium for the detection of MRSA and their resistance rates to other routinely used antibiotics. Additionally, gel-based PCR technique was used for the detection of mecA gene.
Results: Of the subjects under investigation, 200 (26.7%) had Staphylococcus aureus isolates.
MRSA was detected in 86 cases (43%). MRSA was mostly isolated from newly admitted patients (75,6%) and medical personnel (24,4%). The clinical samples with the greatest MRSA prevalence (43.3%) were pus swabs. In 97.6% of cases, chromogenic agar was successfully identified MRSA isolates. All isolates that were resistant to oxacillin disc had positive PCR results.
Conclusion: Using of chromogenic media as a rapid screening method and accurate diagnosis of MRSA strains is depended upon its high sensitivity and specificity compared to PCR method.
Article Type
Original Article
How to Cite This Article
Fahim, Ibrahim Ramadan; Mohamed, Mahmoud Abdel-Hady; Ezzat, Amgad Ahmed Meshref; and Alkherkhisy, Mohammad Mostafa
(2023)
"Evaluation of a Chromogenic Methicillin Resistant Staphylococcus Aureus Selective Medium,"
Al-Azhar International Medical Journal: Vol. 4:
Iss.
9, Article 11.
DOI: https://doi.org/10.58675/2682-339X.2030
